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Cultural characters Of Vibriocholerae

 

Cultural characters Of Vibriocholerae

 

•The ph range at which vibrios grow is 8.5-9.5 .

•They are killed by acid

•On non selective media vibrio produces

– convex smooth colonies that are opaque and granular

•The vibrio cholera can grow over a wide range of temperature

– the optimum temperature is 37oC.

Can grow on a wide variety of culture media

–Simple

–Enriched

–Selective

– chemically defined media containing asparagine and mineral salts as sources of nitrogen and carbon.

•Vibrio cholerae produces oxidase.The oxidase test differentiates it from enterobacteriacae

Susceptibility to compound 0/129 differentiates it from aremonas.vibrio is sensitive to O/129 .Aremonas is  resistant

 

Thiosulphate citrate bile sucrose medium(TCBS)-vibrios can grow on this selective medium.produce yellow sucrose fermenting colonies –oxidase positive

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