Cultural characters Of Vibriocholerae
•The ph range at which vibrios grow is 8.5-9.5 .
•They are killed by acid
•On non selective media vibrio produces
– convex smooth colonies that are opaque and granular
•The vibrio cholera can grow over a wide range of temperature
– the optimum temperature is 37oC.
•Can grow on a wide variety of culture media
– chemically defined media containing asparagine and mineral salts as sources of nitrogen and carbon.
•Vibrio cholerae produces oxidase.The oxidase test differentiates it from enterobacteriacae
•Susceptibility to compound 0/129 differentiates it from aremonas.vibrio is sensitive to O/129 .Aremonas is resistant
•Thiosulphate citrate bile sucrose medium(TCBS)-vibrios can grow on this selective medium.produce yellow sucrose fermenting colonies –oxidase positive